1-deoxy-d-xylulose 5-phosphate Synthase: a Novel Random Sequential Mechanism in Thiamine Diphosphate-dependent Enzymology
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چکیده
Background: 1-Deoxy-D-xylulose 5-phosphate (DXP) synthase is a thiamine diphosphate (ThDP)dependent enzyme in pathogen isoprenoid biosynthesis and a potential drug target. Results: Tryptophan fluorescence and kinetic analyses show that donor and acceptor substrates bind reversibly and independently to DXP synthase. Conclusion: DXP synthase catalyzes a novel, ThDP-dependent, random sequential mechanism. Significance: Targeting the unique kinetic mechanism of DXP synthase could lead to new anti-infective agents.
منابع مشابه
Mechanistic Studies of 1-Deoxy-D-Xylulose-5-Phosphate Synthase from Deinococcus radiodurans
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The enzyme 1-deoxy-d-xylulose 5-phosphate synthase (DXPS) is a key enzyme in the methylerythritol 4-phosphate pathway and is a target for the development of antibiotics, herbicides, and antimalarial drugs. DXPS catalyzes the formation of 1-deoxy-d-xylulose 5-phosphate (DXP), a branch point metabolite in isoprenoid biosynthesis, and is also used in the biosynthesis of thiamin (vitamin B1) and py...
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تاریخ انتشار 2011